CRISPR Screen Dataset
Gao Y (2023) - 2-PMID36693839
Title: A CRISPR-Cas9 screen identifies EXO1 as a formaldehyde resistance gene.
Screen Details:
- Screen Rationale : Increased sensitivity to drug
- Cell Type : Retinal Pigment Epithelium Cell Line [BTO:0002334]
- Cell Line : hTERT-RPE1 [BTO:0004790, CELLOSAURUS:CVCL_4388]
- Phenotype : Response To Chemicals
- Condition : Formaldehyde (70.0 µM) [PubChem]
- Library : TKO (Toronto Knockout) v1 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 22 | Full Dataset Size: 17,227
- Experimental Setup : Drug Exposure | Duration: 15 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Z-score) < -3.0 AND Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in decreased proliferation in the presence of formaldehyde, suggesting that loss of the gene causes increased sensitivity and the gene normally plays a protective role in formaldehyde exposure
Score Distribution
< 13.26
> -16.58