CRISPR Screen Dataset
Ngo AM (2023) - 2-PMID36971544
Title: Genome-Scale Analysis of Cellular Restriction Factors That Inhibit Transgene Expression from Adeno-Associated Virus Vectors.
Screen Details:
- Screen Rationale : Restriction factors of rAAV transgene expression
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Spin infection with AAV2-GFP (50000.0 vg/cell (viral genomes/cell))
- Library : CRISPRi-v2 (inhibition) [AddGene] | Type: CRISPRi | Format: Pool | Enzyme: dCas9-KRAB | Methodology: Inhibition
- Analysis Method : MaGeCK | Number of Hits: 88 | Full Dataset Size: 18,828
- Experimental Setup : Other | Duration: 12 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.1 (MaGeCK Score) < 1e-05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors used an inhibition library to screen for genes whose loss results in increased expression of AAV2-GFP, suggesting the gene normally functions in restricting rAAV transgene expression
Score Distribution
< 1
> 2.57e-17