CRISPR Screen Dataset
Shi Y (2023) - 1-PMID37024475
Title: PRMT3-mediated arginine methylation of IGF2BP1 promotes oxaliplatin resistance in liver cancer.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Hepatoma Cell Line [BTO:0000578]
- Cell Line : Hep-G2 [BTO:0000599, CELLOSAURUS:CVCL_0027]
- Phenotype : Response To Chemicals
- Condition : Oxaliplatin (2.0 µM) [ChEBI]
- Library : Human SAM library in lenti-puro backbone [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: dCas9 | Methodology: Activation
- Analysis Method : MaGeCK | Number of Hits: 761 | Full Dataset Size: 23,430
- Experimental Setup : Drug Exposure | Duration: 7 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.4 (Log2FC) >= 1.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Activation of the target gene results in increased growth in the presence of oxaliplatin, suggesting the gene plays a role in resistance
Score Distribution
< 0.046082
> 0.00000623