CRISPR Screen Dataset
Li M (2023) - 1-PMID37075745
Title: Epigenetic Control of Translation Checkpoint and Tumor Progression via RUVBL1-EEF1A1 Axis.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Rhabdomyosarcoma Cell Line [BTO:0002267]
- Cell Line : A-673 [BTO:0003768, CELLOSAURUS:CVCL_0080]
- Phenotype : Cell Proliferation
- Library : Epigenetic targeted CRISPRi library (Chen, 2023) | Type: CRISPRi | Format: Pool | Enzyme: dCas9-KRAB | Methodology: Inhibition
- Analysis Method : MaGeCK | Number of Hits: 85 | Full Dataset Size: 729
- Experimental Setup : Timecourse | Duration: 16 Days
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Inhibition of the hit genes results in decreased proliferation in A-673 cells suggesting the gene in essential to cell growth
Score Distribution
< 5.2204
> 0