CRISPR Screen Dataset
Deng L (2023) - 1-PMID36725843
Title: Identifying CDC7 as a synergistic target of chemotherapy in resistant small-cell lung cancer via CRISPR/Cas9 screening.
Screen Details:
- Screen Rationale : Increased sensitivity to drug
- Cell Type : Small Cell Lung Cancer [BTO:0000189]
- Cell Line : NCI-H69AR [CELLOSAURUS:CVCL_3513]
- Phenotype : Response To Chemicals
- Condition : Cisplatin|Etoposide (1.0 µg/mL) [ChemSpider, ChEBI]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 194 | Full Dataset Size: 20,540
- Experimental Setup : Drug Exposure | Duration: 7 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Log2FC) <= -2.0 AND Score.2 (p-Value) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in decreased proliferation in the presence of cisplatin (DDP, 1 ug/ml) and etoposide (VP16, 50 ug/ml ), suggesting KO of the gene sensitizes SCLC cells to chemotherapy
Score Distribution
< 9.1497
> -10.229