CRISPR Screen Dataset
Wang S (2025) - 1-PMID40632661
Title: Genome-Wide CRISPR-Cas9 Knockout Screening Identifies Genes Modulating Cisplatin-Induced Cytotoxicity in Renal Proximal Tubule Epithelial Cells.
Screen Details:
- Screen Rationale : Increased resistance to drug
- Cell Type : Kidney Cell Line [BTO:0000067]
- Cell Line : HK-2 [BTO:0003575, CELLOSAURUS:CVCL_0302]
- Phenotype : Response To Chemicals
- Condition : Cisplatin (3.0 µg/mL) [ChemSpider]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 111 | Full Dataset Size: 17,459
- Experimental Setup : Drug Exposure | Duration: 5 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.25 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in increased growth in the presence of cisplatin, suggesting the gene contributes to cisplatin sensitivity
Score Distribution
< 1
> 0.0000437