CRISPR Screen Dataset
Bellail AC (2021) - 1-PMID34644148
Title: Ubiquitination and degradation of SUMO1 by small-molecule degraders extends survival of mice with patient-derived tumors.
Screen Details:
- Screen Rationale : Increased resistance to drug
- Cell Type : Colorectal Cancer Cell Line [BTO:0001616]
- Cell Line : HCT 116 [BTO:0001109, CELLOSAURUS:CVCL_0291]
- Phenotype : Response To Chemicals
- Condition : HB007 (2.5 µM) [ChemSpider]
- Library : CRISPRn (Hao, 2021) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 28 | Full Dataset Size: 20,664
- Experimental Setup : Drug Exposure | Duration: 21 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (FDR) < 0.053 AND Score.2 (Log2FC) > 0.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR knockout screen was carried out to identify genes that rendered HCT 116 cells resistant to HB007 treatment.
Score Distribution
< 1
> 0.00165