CRISPR Screen Dataset
Wang C (2019) - 3-PMID30532030
Title: Genome-wide CRISPR screens reveal synthetic lethality of RNASEH2 deficiency and ATR inhibition.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Mammary Epithelial Cell Line [BTO:0002768]
- Cell Line : MCF-10A [BTO:0001939, CELLOSAURUS:CVCL_0598]
- Phenotype : Response To Chemicals
- Condition : AZD6738 (20.0 IC) [PubChem]
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 624 | Full Dataset Size: 17,730
- Experimental Setup : Drug Exposure | Duration: 21 Days | MOI: < 0.3
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 OR Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant negative DrugZ score show increased sensitivity to ATRi upon KO
- Genes with a significant positive DrugZ score show decreased sensitivity to ATRi upon KO
Score Distribution
< 27.06
> -32.9