CRISPR Screen Dataset
Montoya VR (2023) - 3-PMID36744886
Title: A Virus-Packageable CRISPR System Identifies Host Dependency Factors Co-Opted by Multiple HIV-1 Strains.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : T-lymphoblastic Leukemia Cell Line [BTO:0002504]
- Cell Line : Jurkat [BTO:0000661, CELLOSAURUS:CVCL_0065]
- Phenotype : Response To Virus
- Condition : Virus: HIV (Human immunodeficiency virus) (0.5 MOI) [NCBI Taxid 12721]
- Library : HIVDEP | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 20 | Full Dataset Size: 1,054
- Experimental Setup : Virus Exposure | Duration: 3 Days | MOI: < 1.0
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.002 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit genes results in less productive infection by the HIV-1 LAI strain in Jurkat-CCR5 cells, suggesting that the gene is a host dependency factor for infection by the HIV-LAI strain
- HIVDEP library based on results of genome-wide screen
Score Distribution
< 0.99978
> 0.000000000909