CRISPR Screen Dataset
Al Adhami H (2023) - 1-PMID36772830
Title: Systematic identification of factors involved in the silencing of germline genes in mouse embryonic stem cells.
Screen Details:
- Screen Rationale : Germline gene silencing factors
- Cell Type : Embryonic Cell Line [EFO:0003040]
- Cell Line : Mouse Embryonic Stem Cell [EFO:0004038]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 126 | Full Dataset Size: 19,673
- Experimental Setup : Timecourse | Duration: 10 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (p-Value) < 0.005 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene led to increased expression of germline genes suggesting the gene normally functions in silencing of germline gene expression
- Authors used a cell line expressing GFP under the control of the endogenous promoter of the germline gene Dazl as a reporter and assayed for increased GFP expression
Score Distribution
< 1
> 0.000000252