CRISPR Screen Dataset
Jeon Y (2023) - 1-PMID36977592
Title: FBXO11 governs macrophage cell death and inflammation in response to bacterial toxins.
Screen Details:
- Screen Rationale : Increased resistance to toxin
- Cell Type : Monocytic Leukemia Cell Line [BTO:0002332]
- Cell Line : THP-1 [BTO:0001370, CELLOSAURUS:CVCL_0006]
- Phenotype : Response To Toxin
- Condition : Panton–Valentine leukocidin (PVL) (2.0 µg/mL)
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,178 | Full Dataset Size: 19,114
- Experimental Setup : Toxin Exposure | Duration: 24 Hours | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in increased proliferation in the presence of Panton-Valentine leukocidin (PVL) compared to control
Score Distribution
< 0.99996
> 0.00000000936