CRISPR Screen Dataset
Chen Z (2024) - 1-PMID39547224
Title: PRDX6 contributes to selenocysteine metabolism and ferroptosis resistance.
Screen Details:
- Screen Rationale : Selenocysteine-dependent regulation of ferroptosis
- Cell Type : Neuroblastoma Cell Line [BTO:0000932, EFO:0005214]
- Cell Line : SK-N-DZ SCLY-deficient cells (SCLY-KO)
- Phenotype : Viability [APO:0000111]
- Condition : L-Selenocystine (50.0 nM) [ChemSpider]
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1 | Full Dataset Size: 18,052
- Experimental Setup : Timecourse | Duration: 14 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A loss-of-function genome-wide CRISPR screen was carried out in a clonal selenocysteine lyase knockout (SCLY-KO) SK-N-DZ cell line to identify alternative pathways that could promote selenocysteine-dependent selenoprotein production without SCLY. The experiment involved exposure to Lipoxitatine-1 = 500nM and L-Selenocystine = 50nM.
Score Distribution
< 1
> 0.00495