CRISPR Screen Dataset
Guo R (2020) - 2-PMID32424339
Title: DNA methylation enzymes and PRC1 restrict B-cell Epstein-Barr virus oncoprotein expression.
Screen Details:
- Screen Rationale : Suppression of EBV latency III phase
- Cell Type : Burkitt Lymphoma Cell Line [BTO:0000889]
- Cell Line : Mutu-1 [CELLOSAURUS:CVCL_7202]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : STARS | Number of Hits: 4 | Full Dataset Size: 19,114
- Experimental Setup : Timecourse | Duration: 8 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for genes whose KO resulted in increased markers of latency III (ICAM-1high/CD10high) cells, suggesting the gene normally plays a role in repressing latency III
Score Distribution
< 11.96797089
> 0.535328417