CRISPR Screen Dataset
Le Pen J (2024) - 1-PMID39316623
Title: A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist.
Screen Details:
- Screen Rationale : Increased/decreased resistance to virus
- Cell Type : Huh-7 Cell [BTO:0001950]
- Cell Line : Huh-7.5 [BTO:0004126, CELLOSAURUS:CVCL_7927]
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) (0.3 MOI) [NCBI Taxid 2697049]
- Library : Edit-R Human Whole Genome crRNA Library | Type: CRISPRn | Format: Array | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Z-score | Number of Hits: 557 | Full Dataset Size: 12,119
- Experimental Setup : Virus Exposure | Duration: 6 Days
- Significance : Authors have indicated that results with Score.1 (Z-score) >= 2.0 OR Score.1 (Z-score) <= -2.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a SARS-CoV-2 infection z-score >=2 are antiviral hits and genes with a SARS-CoV-2 infection z-score <=-2 are proviral hits
Score Distribution
< 10.68634478
> -12.33783447