CRISPR Screen Dataset

Llorca-Cardenosa MJ (2022) - 1-PMID36273494

H. sapiens

Title: SMG8/SMG9 Heterodimer Loss Modulates SMG1 Kinase to Drive ATR Inhibitor Resistance.

Screen Rationale : Increased drug resistance
Cell Type : Gastric Cancer Cell Line [BTO:0000787]
Cell Line : YCC-6 [CELLOSAURUS:CVCL_9662]
Phenotype : Response To Chemicals
Condition : Berzosertib (VE822) (100.0 nmol/L) [PubChem]
Library : Human Improved Genome-wide Knockout CRISPR Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : Z-score | Number of Hits: 559 | Full Dataset Size: 16,382
Experimental Setup : Drug Exposure | Duration: 3 Weeks | MOI: = 0.3
Significance : Authors have indicated that results with Score.1 (Z-score) > 2.0 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

KO of hit genes results in increased resistance to ATR inhibition (berzosertib) in gastric cancer cells

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