CRISPR Screen Dataset
Zelceski A (2023) - 1-PMID37163373
Title: MND1 and PSMC3IP control PARP inhibitor sensitivity in mitotic cells.
Screen Details:
- Screen Rationale : Increased sensitivity to drug
- Cell Type : Mammary Epithelial Cell Line [BTO:0002768]
- Cell Line : MCF-10A [BTO:0001939, CELLOSAURUS:CVCL_0598]
- Phenotype : Response To Chemicals
- Condition : Olaparib (2.5 µM) [PubChem]
- Library : Human Improved Genome-wide Knockout CRISPR Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Z-score | Number of Hits: 879 | Full Dataset Size: 18,009
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Z-score) < -3.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in decreased proliferation in the presence of olaparib in MCF10A TP53-/- cells
Score Distribution
< 9.122376074
> -16.91669802