CRISPR Screen Dataset
Beetham H (2022) - 1-PMID34921014
Title: Loss of Integrin-Linked Kinase Sensitizes Breast Cancer to SRC Inhibitors.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Breast Adenocarcinoma Cell Line [BTO:0001912]
- Cell Line : MDA-MB-231 [BTO:0000815, CELLOSAURUS:CVCL_0062]
- Phenotype : Response To Chemicals
- Condition : bosutinib (0.9 μmol/L) [ChEBI]
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 42 | Full Dataset Size: 18,053
- Experimental Setup : Drug Exposure | Duration: 27 Days | MOI: = 0.36
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 OR Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a negative Z-score show increased sensitivity to bosutinib upon KO, hit genes with a positive Z-score show decreased sensitivity to bosutinib upon KO
Score Distribution
< 6.33
> -7.65