CRISPR Screen Dataset
Liu Y (2022) - 1-PMID36396656
Title: mTOR inhibition attenuates chemosensitivity through the induction of chemotherapy resistant persisters.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Pancreatic Ductal Adenocarcinoma Cell Line [BTO:0003883]
- Cell Line : 4292 KRASG12D/p53R172H
- Phenotype : Response To Chemicals
- Condition : Gemcitabine (20.0 nM) [ChEBI]
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,303 | Full Dataset Size: 19,674
- Experimental Setup : Drug Exposure | Duration: 12 Days
- Significance : Authors have indicated that results with Score.1 (Beta Score) > 0.643 OR Score.1 (Beta Score) < -0.575 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with significant positive beta score show increased resistance to gemcitabine upon KO
- Genes with significant negative beta score show increased sensitivity to gemcitabine upon KO
Score Distribution
< 4.8147
> -2.0205