CRISPR Screen Dataset
Tuano NK (2023) - 39-PMID36991492
Title: CRISPR screens identify gene targets at breast cancer risk loci.
Screen Details:
- Screen Rationale : Tumor promoting and suppressing genes
- Cell Type : Mammary Epithelial Cell Line [BTO:0002768]
- Cell Line : HMEC
- Phenotype : Tumorigenicity
- Condition : Mutation: MAP2K1 (ETG5604) constitutively active form of MEK1 (MEKDD)
- Library : CRISPRn targeted BC risk gene library (Rosenbluh, 2023) | Type: CRISPRn | Format: in vivo | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 232 | Full Dataset Size: 1,948
- Experimental Setup : Implantation to Mouse Model | Duration: 42 Days | MOI: = 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant positive log2fc cause increased tumor formation upon knockout, suggesting the gene plays a tumor suppressing role
- Genes with a significant negative log2fc cause decreased tumor formation upon knockout, suggesting the gene plays an oncogenic role
- screen performed in HMEC cells with a luminal progenitor signature (K5+/K19+) implanted into a mouse model
- authors used a cut-off of -1>log2fc>1 and log10FDR>1
Score Distribution
< 15.318
> -8.0922