CRISPR Screen Dataset
Tuano NK (2023) - 27-PMID36991492
Title: CRISPR screens identify gene targets at breast cancer risk loci.
Screen Details:
- Screen Rationale : Increased tumor cell proliferation upon activation
- Cell Type : Mammary Epithelial Cell Line [BTO:0002768]
- Cell Line : HMEC
- Phenotype : Cell Proliferation
- Library : CRISPRa targeted BC risk gene library (Rosenbluh, 2023) | Type: CRISPRa | Format: Pool | Enzyme: dCAS-VP64_Blast (Zhu, 2021) | Methodology: Activation
- Analysis Method : MaGeCK | Full Dataset Size: 1,960
- Experimental Setup : Timecourse | Duration: 21 Days | MOI: = 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant positive log2fc cause increased proliferation upon activation, suggesting the gene plays an oncogenic role
- screen performed in HMEC cells with a mesenchymal signature (B80-T17)
- authors used a cut-off of log2fc>1 and log10FDR>1
Score Distribution
< 0.85477
> -5.7809