CRISPR Screen Dataset
Tuano NK (2023) - 10-PMID36991492
Title: CRISPR screens identify gene targets at breast cancer risk loci.
Screen Details:
- Screen Rationale : Cell-essential and growth restrictive genes
- Cell Type : Mammary Epithelial Cell Line [BTO:0002768]
- Cell Line : HMEC
- Phenotype : Cell Proliferation
- Library : CRISPRn targeted BC risk gene library (Rosenbluh, 2023) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 297 | Full Dataset Size: 1,948
- Experimental Setup : Timecourse | Duration: 21 Days | MOI: = 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant positive log2fc cause increased proliferation upon knockout, suggesting the gene plays a role in tumor suppression
- Genes with a significant negative log2fc cause decreased proliferation upon knockout, suggesting the gene plays an oncogenic role
- screen performed in HMEC cells with an epithelial like signature (B80-T5)
- authors used a cut-off of -1>log2fc>1 and log10FDR>1
Score Distribution
< 1.42
> -6.05