CRISPR Screen Dataset
Kwanten B (2023) - 2-PMID36731340
Title: E3 ubiquitin ligase ASB8 promotes selinexor-induced proteasomal degradation of XPO1.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Chronic Myelogenous Leukemia Cell Line []
- Cell Line : HAP-1 [EFO:0007598, CELLOSAURUS:CVCL_Y019]
- Phenotype : Response To Chemicals
- Condition : selinexor (100.0 nM) [ChEBI]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CRISPRBetaBinomial | Number of Hits: 1,411 | Full Dataset Size: 19,115
- Experimental Setup : Drug Exposure | Duration: 24 Days | MOI: ~ 0.25
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a positive lfc show increased resistance to selinexor
- Hit genes with a negative lfc show increased sensitivity to selinexor
Score Distribution
< 5.99131107
> -3.142554089