CRISPR Screen Dataset
Liao S (2017) - 1-PMID28167502
Title: A genetic interaction analysis identifies cancer drivers that modify EGFR dependency.
Screen Details:
- Screen Rationale : Increased drug resistance
- Cell Type : Lung Adenocarcinoma Cell Line [BTO:0001911]
- Cell Line : PC-9 [BTO:0005518, CELLOSAURUS:CVCL_B260]
- Phenotype : Response To Chemicals
- Condition : Gefitinib (30.0 nM) [ChemSpider, PubChem]
- Library : TSG targeted CRISPRn library (Elledge, 2017) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 34 | Full Dataset Size: 498
- Experimental Setup : Drug Exposure | Duration: 17 Days | MOI: = 0.2
- Significance : Authors have indicated that results with Score.4 (FDR) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit genes results in increased resistance to gefitinib
Score Distribution
< 498
> 1