CRISPR Screen Dataset
Kesner JS (2023) - 1-PMID37046090
Title: Noncoding translation mitigation.
Screen Details:
- Screen Rationale : Regulation of non-coding translation
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Human Activity-optimized genome-wide library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 22 | Full Dataset Size: 18,077
- Experimental Setup : Timecourse | Duration: 48 Hours
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.01 OR Score.4 (FDR) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- authors used an AMD1 reporter to measure changes in readthrough translation in the cell
Score Distribution
< 0.99997
> 0.00000000000865