CRISPR Screen Dataset
Belk JA (2022) - 1-PMID35750052
Title: Genome-wide CRISPR screens of T cell exhaustion identify chromatin remodeling factors that limit T cell persistence.
Screen Details:
- Screen Rationale : Regulation of T cell exhaustion
- Cell Type : Regulatory T Cell [CL:0000815]
- Cell Line : CD8+ T s (primary-derived)
- Phenotype : Cell Proliferation
- Condition : Chronic T cell stimulation
- Library : Teichmann Retroviral Mouse Genome-wide CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Z-score | Number of Hits: 99 | Full Dataset Size: 17,447
- Experimental Setup : Other | Duration: 10 Days
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.06 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a positive z-score are enriched in chronically stimulated T cells as opposed to acutely stimulated T-cells
Score Distribution
< 21.13709118
> -5.835819176