CRISPR Screen Dataset
van der Weegen Y (2021) - 4-PMID34108663
Title: ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance in an ELOF1-KO background
- Cell Type : Retinal Pigment Epithelium Cell Line [BTO:0002334]
- Cell Line : hTERT-RPE1 [BTO:0004790, CELLOSAURUS:CVCL_4388]
- Phenotype : Response To Chemicals
- Condition : Mutation: ELOF1 (ETG84337) KO| Illudin S (5.0 nM)
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: iCAS9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 45 | Full Dataset Size: 17,822
- Experimental Setup : Drug Exposure | Duration: 12 Doublings | MOI: ~ 0.2
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.01 OR Score.3 (FDR) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a negative Z-score show increased sensitivity to Illudin S upon KO in an ELOF1 mutant background
- Hit genes with a positive Z-score show increased resistance to Illudin S upon KO in an ELOF1 mutant background
Score Distribution
< 12.63
> -11.42