CRISPR Screen Dataset

van der Weegen Y (2021) - 3-PMID34108663

H. sapiens

Title: ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation.

Screen Rationale : Increased/decreased drug resistance in a CSB-KO background
Cell Type : Retinal Pigment Epithelium Cell Line [BTO:0002334]
Cell Line : hTERT-RPE1 [BTO:0004790, CELLOSAURUS:CVCL_4388]
Phenotype : Response To Chemicals
Condition : Mutation: ERCC6 (ETG2074) KO| Illudin S (2.0 nM)
Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: iCAS9 | Methodology: Knockout
Analysis Method : DrugZ | Number of Hits: 138 | Full Dataset Size: 17,819
Experimental Setup : Drug Exposure | Duration: 12 Doublings | MOI: ~ 0.2
Significance : Authors have indicated that results with Score.2 (FDR) < 0.01 OR Score.3 (FDR) < 0.01 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Hit genes with a negative Z-score show increased sensitivity to Illudin S upon KO in a CSB mutant background
Hit genes with a positive Z-score show increased resistance to Illudin S upon KO in a CSB mutant background

< 18.25

> -13.78