CRISPR Screen Dataset
van der Weegen Y (2021) - 1-PMID34108663
Title: ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Retinal Pigment Epithelium Cell Line [BTO:0002334]
- Cell Line : hTERT-RPE1 [BTO:0004790, CELLOSAURUS:CVCL_4388]
- Phenotype : Response To Chemicals
- Condition : Illudin S (25.0 nM) [PubChem]
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: iCAS9 | Methodology: Knockout
- Analysis Method : DrugZ | Number of Hits: 121 | Full Dataset Size: 17,821
- Experimental Setup : Drug Exposure | Duration: 12 Doublings | MOI: ~ 0.2
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.01 OR Score.4 (FDR) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a negative Z-score show increased sensitivity to Illudin S upon KO
- Hit genes with a positive Z-score show decreased sensitivity to Illudin S upon KO
Score Distribution
< 16.21
> -17.33