CRISPR Screen Dataset
Gu X (2023) - 1-PMID37616343
Title: The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation.
Screen Details:
- Screen Rationale : Increased protein stabilization
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Root library (Gu, 2023) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 47 | Full Dataset Size: 19,024
- Experimental Setup : Timecourse | Duration: 9 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (MAGeCK pos score) < 4e-05 AND Score.2 (FDR) < 0.1 AND Score.3 (Log2FC) > 0.5 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A FACS-based genome-wide CRISPR/Cas9 screen was carried out in HEK-293T cells stably expressing a global protein stability (GPS) reporter for EGR1 in order to identify genes whose disruption stabilized EGR1.
Score Distribution
< 1
> 0.00000000000000815