CRISPR Screen Dataset
Chan K (2022) - 5-PMID36597481
Title: Survival-based CRISPR genetic screens across a panel of permissive cell lines identify common and cell-specific SARS-CoV-2 host factors.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : African Green Monkey Kidney Cell Line
- Cell Line : Vero-E6|VERO C1008 [BTO:0004755, COVOC:0020002, CELLOSAURUS:CVCL_0574]
- Phenotype : Cell Proliferation
- Library : Toronto Knockout version 3 (TKOv3) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : BAGEL | Number of Hits: 1,641 | Full Dataset Size: 18,051
- Experimental Setup : Timecourse | Duration: 15 Doublings | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 5.0 AND Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR knockout screen was performed to identify context-essential genes within a cell line and core essential genes across five cell lines. African green monkey Vero-E6 cells were screened using a human Toronto Knockout version 3 (TKOv3) library with 56% of the library mapping with perfect homology to 15, 935 loci in the Vero-E6 genome.
Score Distribution
< 43.575
> -259.988