CRISPR Screen Dataset

Ugalde AP (2022) - 1-PMID36124427

H. sapiens

Title: Autophagy-linked plasma and lysosomal membrane protein PLAC8 is a key host factor for SARS-CoV-2 entry into human cells.

Screen Rationale : Decreased resistance to virus
Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
Cell Line : Calu1-ACE2
Phenotype : Viability [APO:0000111]
Condition : SARS-CoV-2 Spike‐Δ19 protein pseudotyped lentivirus (25.0 %)
Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 104 | Full Dataset Size: 20,914
Experimental Setup : Virus Exposure | Duration: 6 Days | MOI: = 0.3
Significance : Authors have indicated that results with Score.1 (Log2FC) < 0.0 AND Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

A CRISPR/Cas9 genome-wide screen was carried out to identify novel host factors for SARS-CoV-2 entry. A cellular model was used involving Calu-1 cells overexpressing ACE2 and a SARS-CoV-2 Spike-delta 19 protein pseudotyped lentivirus.

< 1.6973

> -1.9217