CRISPR Screen Dataset
Alborzinia H (2023) - 4-PMID37435859
Title: LRP8-mediated selenocysteine uptake is a targetable vulnerability in MYCN-amplified neuroblastoma.
Screen Details:
- Screen Rationale : Synthetic lethality with LRP8-KO
- Cell Type : Fibrosarcoma Cell Line [BTO:0000460]
- Cell Line : HT-1080 [BTO:0001282, CELLOSAURUS:CVCL_0317]
- Phenotype : Viability [APO:0000111]
- Condition : LRP8-Knockout (LRP8-KO)
- Library : Superti-Furga Lab Human SLC KO Library | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeckFlute | Number of Hits: 3 | Full Dataset Size: 415
- Experimental Setup : Timecourse | Duration: 14 Days | MOI: = 0.5
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A targeted CRISPRn screen was carried out in HT-1080 cells to identify genes generating synthetic lethality with LRP8 deficiency.
Score Distribution
< 0.73537
> -3.3206