CRISPR Screen Dataset
Zhu Q (2021) - 1-PMID34373448
Title: AHR mediates the aflatoxin B1 toxicity associated with hepatocellular carcinoma.
Screen Details:
- Screen Rationale : Increased resistance to toxin
- Cell Type : Hepatoma Cell Line [BTO:0000578]
- Cell Line : PLC/PRF/5 [BTO:0003492, CELLOSAURUS:CVCL_0485, DEPMAP:ACH-001318]
- Phenotype : Response To Toxin
- Condition : aflatoxin B1 (AFB1) (8 µM) [ChemSpider]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 129 | Full Dataset Size: 19,115
- Experimental Setup : Toxin Exposure | Duration: 12 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (Beta Score) > 0.0 AND Score.3 (p-Value) < 0.01 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in increased viability in the presence of aflatoxin B1
Score Distribution
< 2.7051
> -5.397