CRISPR Screen Dataset

Sintov E (2022) - 1-PMID36055241

H. sapiens

Title: Whole-genome CRISPR screening identifies genetic manipulations to reduce immune rejection of stem cell-derived islets.

Screen Rationale : Regulation of stem cell (SC)-islet graft response to immune attack
Cell Type : Pancreatic Islet [BTO:0000991]
Cell Line : stem cell-derived islets
Phenotype : Cell Proliferation
Condition : Exposure to allogeneic peripheral blood mononuclear cells (PBMCs)
Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: in vivo | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : FDR | Number of Hits: 654 | Full Dataset Size: 11,689
Experimental Setup : Timecourse | Duration: 10 Weeks | MOI: < 1.0
Significance : Authors have indicated that results with Score.2 (FDR) >= 0.6989 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Hit genes with a positive fold change value increase survival of SC-islet graft cells under immune attack upon KO
Hit genes with a negative fold change value increase sensitivity of SC-islet graft cells to immune attack upon KO

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