CRISPR Screen Dataset
Weiss-Sadan T (2023) - 2-PMID36841242
Title: NRF2 activation induces NADH-reductive stress, providing a metabolic vulnerability in lung cancer.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : Calu-6 [BTO:0002235, CELLOSAURUS:CVCL_0236]
- Phenotype : Response To Chemicals
- Condition : KI-696 (KI696) (1.0 µM) [ChemSpider, PubChem]
- Library : Human CRISPR Metabolic Gene Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Differential CRISPR score | Number of Hits: 5 | Full Dataset Size: 2,978
- Experimental Setup : Drug Exposure | Duration: 11 Doublings | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (CRISPR Score (CS)) >= 2.0 OR Score.1 (CRISPR Score (CS)) <= -2.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened a metabolism targeted library for genes that showed increased resistance (pos CRISPR score) or decreased resistance (neg CRISPR score) to the NRF2 activation drug, KI696, upon KO in a KEAP1-dependent cell line
Score Distribution
< 1.959993551
> -2.849648532