CRISPR Screen Dataset
Tian X (2021) - 3-PMID34026427
Title: A Fifteen-Gene Classifier to Predict Neoadjuvant Chemotherapy Responses in Patients with Stage IB to IIB Squamous Cervical Cancer.
Screen Details:
- Screen Rationale : Increased resistance to drugs
- Cell Type : Cervical Squamous Cell Carcinoma [BTO:0002212]
- Cell Line : SiHa [BTO:0002210, CELLOSAURUS:CVCL_0032, DEPMAP:ACH-000556]
- Phenotype : Response To Chemicals
- Condition : Cisplatin and paclitaxel (taxol) [ChemSpider, ChemSpider]
- Library : Targeted Resistant Cervical Cancer Cell Library (Hu, 2021) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 261 | Full Dataset Size: 744
- Experimental Setup : Drug Exposure | Duration: 14 Days | MOI: = 0.2
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of hit genes results in increased cervical cancer cell resistance to the combination chemotherapy with Cisplatin (10uM) and Taxol (30nM)
Score Distribution
< 19.77886537
> 0.508939004