CRISPR Screen Dataset
Mac Kain A (2022) - 1-PMID35508460
Title: Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen.
Screen Details:
- Screen Rationale : Increased/decreased resistance to virus
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : A549-ACE2
- Phenotype : Response To Virus
- Condition : Virus: SARS-CoV-2 (isolate USA-WA1/2020) (1.0 MOI)
- Library : CRISPRn Targeted 1905 Interferon Stimulated Genes (ISGs) (Emerman, 2018) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 10 | Full Dataset Size: 2,105
- Experimental Setup : Virus Exposure | Duration: 24 Hours | MOI: = 0.1
- Significance : Authors have indicated that results with Score.1 (Log2FC) < 0.0 AND Score.2 (FDR) < 0.05 OR Score.1 (Log2FC) > 0.0 AND Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A targeted CRISPR/Cas9 screen was carried out in A549-ACE2 cells knocked out for 1905 potential interferon stimulated genes (ISGs) to identify ISGs that modulate viral replication in cells infected with SARS-CoV-2. Genes significantly enriched in infected cells represent antiviral genes and depleted ones represent proviral genes.
Score Distribution
< 0.80838
> -1.235