CRISPR Screen Dataset
Yang J (2023) - 2-PMID36724073
Title: Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins.
Screen Details:
- Screen Rationale : Regulation of cell-surface TSPAN8 expression in an SPPL3 KO background
- Cell Type : Cancer Cell Line [EFO:0001639]
- Cell Line : MEC [CELLOSAURUS:CVCL_B270]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Mutation: SPPL3 (ETG121665) KO
- Library : Human Two Plasmid Activity-Optimized CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 832 | Full Dataset Size: 18,543
- Experimental Setup : Timecourse | Duration: 8 Days | MOI: ~ 0.4
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of the hit gene results in rescue of TSPAN8 surface expression in an SPPL3 KO background
Score Distribution
< 0.0099217
> 4.16e-19