CRISPR Screen Dataset
Yang J (2023) - 1-PMID36724073
Title: Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins.
Screen Details:
- Screen Rationale : Regulation of cell-surface TSPAN8 expression
- Cell Type : Cancer Cell Line [EFO:0001639]
- Cell Line : MEC [CELLOSAURUS:CVCL_B270]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Human Improved Genome-wide Knockout CRISPR Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 334 | Full Dataset Size: 18,543
- Experimental Setup : Timecourse | Duration: 8 Days | MOI: ~ 0.4
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of the hit gene results in low TSPAN8 surface expression, suggesting the gene plays a role in tetraspanin surface expression
Score Distribution
< 0.0098517
> 8.56e-24