CRISPR Screen Dataset
Wang B (2023) - 1-PMID37193694
Title: Identification of indocyanine green as a STT3B inhibitor against mushroom α-amanitin cytotoxicity.
Screen Details:
- Screen Rationale : Increased resistance to toxin
- Cell Type : Chronic Myelogenous Leukemia Cell Line []
- Cell Line : HAP-1 [EFO:0007598, CELLOSAURUS:CVCL_Y019]
- Phenotype : Response To Toxin
- Condition : alpha-Amanitin (1.5 µM) [ChEBI]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 542 | Full Dataset Size: 19,110
- Experimental Setup : Toxin Exposure | Duration: 7 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (Log2FC) > 1.0 AND Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A genome-wide CRISPR/Cas9 knockout screen was carried out in the haploid cell line HAP1 to identify genes required for alpha-Amantin (AMA)-induced cell death.
Score Distribution
< 10.669
> -4.9829