CRISPR Screen Dataset
Vervoort SJ (2021) - 3-PMID34004147
Title: The PP2A-Integrator-CDK9 axis fine-tunes transcription and can be targeted therapeutically in cancer.
Screen Details:
- Screen Rationale : Increased resistance to drug
- Cell Type : Monocytic Leukemia Cell Line [BTO:0002332]
- Cell Line : THP-1 [BTO:0001370, CELLOSAURUS:CVCL_0006]
- Phenotype : Response To Chemicals
- Condition : AZD5576 (CDK9i) (150.0 nM)
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 546 | Full Dataset Size: 20,107
- Experimental Setup : Drug Exposure | Duration: 21 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.1 AND Score.4 (sgRNA number) > 3.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in increased proliferation in the presence of the CDK9 inhibitor AZD5576
Score Distribution
< 1
> 3.51e-16