CRISPR Screen Dataset

Vervoort SJ (2021) - 1-PMID34004147

H. sapiens

Title: The PP2A-Integrator-CDK9 axis fine-tunes transcription and can be targeted therapeutically in cancer.

Screen Rationale : Regulation of transcription
Cell Type : Monocytic Leukemia Cell Line [BTO:0002332]
Cell Line : THP-1 [BTO:0001370, CELLOSAURUS:CVCL_0006]
Phenotype : RNA Accumulation [APO:0000224]
Condition : AZD5576 (CDK9i) (170.0 nM)
Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 40 | Full Dataset Size: 20,104
Experimental Setup : Drug Exposure | Duration: 16 Hours | MOI: = 0.3
Significance : Authors have indicated that results with Score.2 (p-Value) < 0.1 AND Score.4 (sgRNA number) > 3.0 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Hit genes were enriched in a population which retained transcription (based on EU incorporation and FACS) in the presence of CDK9i

< 1

> 0.0000033