CRISPR Screen Dataset
Lalonde ME (2023) - 3-PMID36134452
Title: Genome-wide CRISPR screens identify ferroptosis as a novel therapeutic vulnerability in acute lymphoblastic leukemia.
Screen Details:
- Screen Rationale : Cell-essential genes and growth restrictive genes
- Cell Type : Acute Lymphoblastic Leukemia Cell Line [BTO:0002144]
- Cell Line : RCH-ACV [BTO:0003376, CELLOSAURUS:CVCL_1851]
- Phenotype : Cell Proliferation
- Library : Human Improved Genome-wide Knockout CRISPR Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,404 | Full Dataset Size: 18,010
- Experimental Setup : Timecourse | Duration: 14 Doublings | MOI: = 0.3
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- Genes with a negative log-fold change are essential in a B-ALL cell line, genes with a positive log-fold change confer a proliferative advantage upon KO in a B-ALL cell line
Score Distribution
< 2.6215
> -4.9757