CRISPR Screen Dataset
Hagel KR (2022) - 2-PMID36548402
Title: Systematic interrogation of tumor cell resistance to CAR T cell therapy in pancreatic cancer.
Screen Details:
- Screen Rationale : Regulation of cancer cell response to immune system
- Cell Type : Cancer Cell Line [EFO:0001639]
- Cell Line : KP-4 [EFO:0006623, CELLOSAURUS:CVCL_1338]
- Phenotype : Cell Proliferation
- Condition : CAR T cells (1.0 Ratio of effector cells/target cells)
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 532 | Full Dataset Size: 20,080
- Experimental Setup : T cell exposure | Duration: 13 Days | MOI: < 1.0
- Significance : Authors have indicated that results with Score.2 (p-Value) > 1.3 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes with a positive log2fc show enrichment in populations exposed to CAR T cells suggesting KO of the gene confers resistance to T cells
- KO of hit genes with a negative log2fc show depletion in populations exposed to CAR T cells suggesting KO of the gene confers sensitivity to T cells
Score Distribution
< 3.257912565
> -1.574206755