CRISPR Screen Dataset
Kerins MJ (2019) - 1-PMID31010806
Title: Genome-Wide CRISPR Screen Reveals Autophagy Disruption as the Convergence Mechanism That Regulates the NRF2 Transcription Factor.
Screen Details:
- Screen Rationale : Negative regulators of NRF2
- Cell Type : Kidney Cell Line [BTO:0000067]
- Cell Line : HK-2 [BTO:0003575, CELLOSAURUS:CVCL_0302]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Blasticidin (10.0 µg/mL) [ChEBI]
- Library : Human Activity-optimized genome-wide library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Etest | Number of Hits: 273 | Full Dataset Size: 18,663
- Experimental Setup : Timecourse | MOI: = 0.1
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of the hit gene results in increased NRF2 activation of a blasticidin resistance reporter gene
Score Distribution
< 14.63483427
> 0.213656033