CRISPR Screen Dataset
Chen H (2022) - 1-PMID35439318
Title: A Whole-Genome CRISPR Screen Identifies AHR Loss as a Mechanism of Resistance to a PARP7 Inhibitor.
Screen Details:
- Screen Rationale : Increased/decreased drug resistance
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : NCI-H1373 [EFO:0006657, CELLOSAURUS:CVCL_1465]
- Phenotype : Response To Chemicals
- Condition : Atamparib (RBN-2397) (20.0 nM) [PubChem]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 2,429 | Full Dataset Size: 19,114
- Experimental Setup : Drug Exposure | Duration: 7 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 OR Score.4 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes with a significant negative score show increased resistance to RBN-2397 upon KO
- Hit genes with a significant positive score show increased sensitivity to RBN-2397 upon KO
Score Distribution
< 0.99997
> 0.00000000000014