CRISPR Screen Dataset
Drainas AP (2020) - 2-PMID32268084
Title: Genome-wide Screens Implicate Loss of Cullin Ring Ligase 3 in Persistent Proliferation and Genome Instability in TP53-Deficient Cells.
Screen Details:
- Screen Rationale : Increased proliferation in a p53 KO mutant background
- Cell Type : Retinal Pigment Epithelium Cell Line [BTO:0002334]
- Cell Line : hTERT-RPE1 [BTO:0004790, CELLOSAURUS:CVCL_4388]
- Phenotype : Cell Proliferation
- Condition : Mutation: TP53 (ETG:7157) KO
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MEMcrispR | Number of Hits: 128 | Full Dataset Size: 20,890
- Experimental Setup : Timecourse | Duration: 7 Days | MOI: = 0.2
- Significance : Authors have indicated that results with Score.1 (mean fold change) > 2.0 AND Score.2 (p-Value) <= 0.2 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes results in increased proliferation in a p53 KO mutant background
Score Distribution
< 59.56529938
> 0.02273427