CRISPR Screen Dataset
Carnevale J (2022) - 6-PMID36002574
Title: RASA2 ablation in T cells boosts antigen sensitivity and long-term function.
Screen Details:
- Screen Rationale : Increased T-cell proliferation
- Cell Type : T Cell [CL:0000084]
- Cell Line : Primary T-cells
- Phenotype : Cell Proliferation
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Z-score based on MAGECK score | Number of Hits: 707 | Full Dataset Size: 19,362
- Experimental Setup : Timecourse | Duration: 17 Days
- Significance : Authors have indicated that results with Score.1 (ZLFC) > 1.54 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit gene results in enrichment in a highly dividing T-cell population compared to a non-highly dividing population in response to stimulation of proliferation
Score Distribution
< 7.519372133
> -11.95208825