CRISPR Screen Dataset
Carnevale J (2022) - 3-PMID36002574
Title: RASA2 ablation in T cells boosts antigen sensitivity and long-term function.
Screen Details:
- Screen Rationale : Increased resistance to suppression of T-cell proliferation
- Cell Type : T Cell [CL:0000084]
- Cell Line : Primary T-cells
- Phenotype : Cell Proliferation
- Condition : Tacrolimus (5.0 nM) [ChEBI]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Z-score based on MAGECK score | Number of Hits: 955 | Full Dataset Size: 14,790
- Experimental Setup : Drug Exposure | Duration: 17 Days
- Significance : Authors have indicated that results with Score.1 (ZLFC) > 1.54 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of the hit genes results in increased proliferation of T-cells even in the presence of suppressive conditions (Tacrolimus)
Score Distribution
< 5.141590552
> -3.901507696