CRISPR Screen Dataset
Oreskovic E (2022) - 2-PMID35149558
Title: Genetic analysis of cancer drivers reveals cohesin and CTCF as suppressors of PD-L1.
Screen Details:
- Screen Rationale : Negative regulators of PD-L1 expression in the presence of IFNgamma
- Cell Type : Mammary Epithelial Cell Line [BTO:0002768]
- Cell Line : HMEC
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Interferon gamma (10.0 ng/mL)
- Library : Tumor Suppressor Gene (TSG) targeted library (Elledge, 2022) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 25 | Full Dataset Size: 498
- Experimental Setup : Cytokine exposure | Duration: 24 Hours | MOI: > 0.2
- Significance : Authors have indicated that results with Score.1 (MaGeCK Score) < 0.0016 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes are those whose KO results in high PD-L1 expression, suggesting the gene normally negatively regulates PD-L1 expression
Score Distribution
< 1
> 2.28e-17